Concordia IT
DIFF Measurement
◼ Reagents:
DIFF reagent: used for lysing of red blood cells and specialization of different white blood cells;
Diluent: background solution used for providing sheath fluid and cleansing
◼ Measurement principle: flow cytometry and laser scatter
◼ Measurement parameters: MONO#, MONO%, LYMPH#, LYMPH %, NEUT#, NEUT%, EOS#, EOS%
◼ Graphics: 4 Differential scattergram
◼ Dilution ratio: 1:105.8
◼ Counting duration: 9.1 s
◼ Counting flow: 0.008575417ml/s
◼ Counting volume: the flow of the sample stream is constant, which can be converted to counting volume by controlling the counting duration
◼ Function description: Mix 15 μL of blood sample and 550 μL of diluent in the WBC bath. After the secondary aspiration, add 1 mL of DIFF lyse. After the reaction has been on for a certain time, place the prepared sample at the bottom end of the flow cell. The sample will be wrapped by a sheath stream generated by the large volume syringe of the syringe linkage and be pushed by the small volume syringe into the flow cell for the measurement.
7.1.1.2 WBC Count and BASO Measurement
◼ Reagents:
LH lyse: used for lysing red blood cells and platelets and separating the basophiles by volume from the other white blood cells
Diluent: used for providing sheath fluid and cleansing
◼ Measurement principle: flow cytometry and laser scatter
◼ Measurement parameters: WBC, BASO# and BASO%
◼ Graphics: WBC histogram
◼ Dilution ratio: 1:123.8*1
◼ Counting duration: 11.5 s
◼ Counting flow: 0.008575417ml/s